RNA isolation and cDNA synthesis were performed as described. ![]() Human colon cancer cells in triplicate were treated with LPS for 8 h. Fifty-five genes were selected for qPCR analysis (Table 1). The effect of LPS on gene expression was evaluated by quantitative real-time PCR analysis (qPCR). Cell cytotoxicity was determined by spectrophotometer at A570 nm using the MTT based-In Vitro Toxicology Assay Kit (Sigma). MO) (“0” treatment represents the control with 1% DMSO in all treatments). coli serotype K235 and purified by gel filtration (Sigma, St. Cancer cells (0.5 mL) were subcultured at 1 × 10 5 cells/mL density and treated for 2 and 24 h with 0–1000 ng/mL of LPS extracted from E. Human colon cancer cells (COLO 205) were maintained in RPMI-1640 medium (Gibco) containing 10% (v:v) fetal bovine serum, 0.1 million units/L penicillin, 100 mg/L streptomycin, and 2 mmol/L l-glutamine at 37 ☌ with 5% CO 2. The results showed that LPS had minimal effect on cell viability but had a profound effect on gene expression at the mRNA levels in the human colon cancer cells. The 55 molecular targets belong to several important pathways, whose expression is affected by the plant toxin gossypol in cancer cells and macrophages or regulated by ZFP36/TTP in tumor cells and macrophages, as well as cinnamon polyphenol extract (Table 1). The objective of this study was to survey the effect of LPS on cell viability and gene expression of 55 molecular targets in human colon cancer cells. LPS induced TGFβ and HGF production, promoted NFkB (NFkappaB) activation and increased the migratory capacity in colon cancer cells. A number of studies explored the effect of LPS on gene expression in colon cancer cells, but they were focused on a few targets in the reported research. LPS was proposed to have antitumor effect in several experimental models. LPS is a heat-stable endotoxin which normally protects gram-negative bacteria against bile salts and lipophilic antibiotics. Intact LPS is made up of three structural components : a hydrophobic lipid section a hydrophilic core polysaccharide chain, and a repeating hydrophilic O-antigenic oligosaccharide side chain. Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. It is urgently needed to fully understand the mechanism of developing colon cancer and explore ways to ease the burden of the healthcare crisis. ![]() The risk of developing colorectal cancer is approximately 4.0% for men and women in 2021 during the lifetime ( ). Colon cancer is one of the deadliest diseases in the World.
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